| Primary Identifier | MGI:7467448 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A viral splice acceptor (SA)-loxP-neomycin-pA-loxP-H2B-mRuby3-P2A-ERK-KTR-mClover3-WPRE-pA cassette is introduced to the mouse Gt(ROSA)26Sor locus. The targeting vector incudes. DNA fragments containing mClover3 (Addgene, #(a href="https://www.addgene.org/74252/" target="_blank")74252(/a)), mRuby3 (Addgene, #(a href="https://www.addgene.org/74252/" target="_blank")74252(/a)), human H2B (Addgene, #(a href="https://www.addgene.org/99267/" target="_blank")99267(/a)), an adenovirus splice acceptor, a floxed NeoR sequence, the ERK-KTR reporter (Addgene, #(a href="https: //www.addgene.org/59138/" target="_blank")59138(/a)), and woodchuck hepatitis virus post-transcriptional regulatory element. The ERK-KTR sensor consists of an ERK1/2 binding site and phosphorylation motif adjacent to phosphorylation activated nuclear export signal (NES) and inhibited nuclear localization sequence (NLS) fused to a fluorescent protein (FP; mClover3). |
