| Primary Identifier | MGI:7471382 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Fgf8 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting construct inserted an IRES sequence followed by the mER:Cre:mER cDNA encoding a fusion protein with two copies of the mouse estrogen receptor (mER) flanking Cre recombinase, and a Neomycin resistance (NeoR) selection cassette flanked by Frt recombination sites into an Eag1 site in the Fgf8 gene located 36 bp 3â² of the Fgf8 stop codon and 5â² to the endogenous polyadenylation sequence. The linearized targeting vector was electroporated into mouse embryonic stem cells and subjected to selection. Flp-recombinase excised the NeoR. |
