| Primary Identifier | MGI:7471434 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Rpl3 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology was used to delete exon 2. Reverse transcription and quantitative PCR analysis of heart and skeletal muscle confirmed that mRNA is either undetectable for the deleted region or reduced in abundance for the 3âUTR, likely due to nonsense-mediated mRNA decay. Mass spectrometry-based multiple reaction monitoring confirmed absence of protein in the heart. |
