| Primary Identifier | MGI:7482002 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inducible, Reporter, Transactivator | Gene | Igs7 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector has a Tet response element (tetO), loxP-STOP-loxP cassette and a Ace2N-4AA-mNeonGreen-WPRE sequence that is followed by a CAG promoter, Lox2272-STOP-Lox2272 cassette, mRuby3, and IRES-tTA2-WPRE sequence - all inserted into the T1 TIGRE locus (an intergenic region on chromosome 9 [between AB124611 and Carm1] determined to have low basal transcriptional activity and allow high cre and/or Tet inducibility). Ace2N (rho*)-4AA-mNeonGreen is an indicator variant of Acetabularia acetabulum-derived rhodopsin (Ace) with an inactivated proton pump (Ace2N), with four extra amino acids in a linker domain that fuses to mNeonGreen fluorescent protein to enable voltage-sensitive fluorescence resonance energy transfer (FRET). Of note, the Ai218 allele was designed with an IRES upstream of the tTA2 to reduce/eliminate leakiness in the absence of cre recombinase. |
