| Primary Identifier | MGI:7488473 | Allele Type | Endonuclease-mediated |
| Attribute String | Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | 6NK7 embryonic stem cells are used |
| molecularNote | Using the CRISPR/Cas9 method, a targeting construct consisting of an AS-loxP/NeoSTOP/loxP-murine Mecom cDNA followed by IRES-EGFP-pA fusion gene inserted into intron 1 of the ROSA26 locus into STOP-eGFP-ROSA26TV plasmid was injected into 6NK7 embryonic stem cells. Mecom and EGFP are expressed after recombination by Cre recombinase driven by endogenous Gt(ROSA)26Sor promoter. The lines containing this allele showed a low recombination rate. |
