| Primary Identifier | MGI:7509136 | Allele Type | Endonuclease-mediated |
| Gene | Trem2 | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology introduced a stop-codon (TGA) after the amino acid histidine 157 into the cleavage-site of TREM2. In addition, a restriction enzyme site Eco57I (CACTGAAGC) replaced the wild-type amino acids histidine, serine and threonine (HST; CACAGCACC). FACS confirmed absence of protein on the cell surface of bone marrow derived macrophages of homozygotes and soluble TREM2 levels are highly reduced. RNA levels are reduced in brain and may account for the reduced soluble protein levels. |
