| Primary Identifier | MGI:7509492 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(17Scaf8-Pde10a)2Yey |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Chromosome-engineering cassettes were inserted into mouse Chromosome 17, bracketing a span between the SR-related CTD-associated factor 8 (Scaf8) gene and the phosphodiesterase 10A (Pde10a) gene. The cassette placed at the proximal locus (MICER clone MHPN155l15) was targeted upstream of Scaf8 and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. The cassette placed at the distal locus (MICER clone MHPP338g19) was targeted downstream of the Pde10a gene and contained an agouti transgene, a 3; portion of an HPRT minigene, a loxP site and puromycin resistance gene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231. |
