Primary Identifier | MGI:7494106 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Il3 |
Transmission | Germline | Strain of Origin | BALB/cN |
Is Recombinase | false | Is Wild Type | false |
molecularNote | IMPC targeting vector ETPG00275_W_2_F02 was modified to remove the lacZ and insert EGFP cDNA. The resulting vector contains an FRT site, En2 splice acceptor, LF2A sequence, EGFP, loxP site, neomycin resistance cassette, FRT site, and loxP site were inserted into intron 2 and third lox P site was inserted downstream of exon 5. The EGFP and neomycin resistance cassette were removed via flp-mediated recombination leaving exons 3-5 floxed. |