| Primary Identifier | MGI:7530769 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Pf4 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector was designed to insert an FRT-flanked neomycin resistance (neo) cassette and a loxP site upstream of exon 2. A second loxP site was inserted downstream of a mutated exon 3. Exon 3 was altered to contain three amino acid substitutions: proline to leucine (P64L), lysine to glutamate (K72E), and isoleucine to histidine (I73H) to obtain the murine orthologue of human CXCL4L1. Flp-mediated recombination removed the neo cassette. |
