| Primary Identifier | MGI:7539829 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Nfx1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTCAGAATGTACGATAACAT and GTTGTCGCTTAACAAGAAGG, which resulted in a 313 bp deletion beginning at Chromosome 4 position 40,988,503 bp and ending after 40,988,815 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000344981 (exon 5) and 207 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 417 and early truncation 45 amino acids later. |
