| Primary Identifier | MGI:7525599 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence | Gene | Fabp4 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The murine Fabp4 sequence was humanized by introducing point mutations to convert the 11 amino acids in exons 2 and 3 that differ between mouse and human FABP4 to human FABP4 sequences. A loxP site was inserted within intron 1 upstream of exon 2 and a FRT-neomycin-FRT-loxP cassette was inserted at the 3â² end of exon 2. Resulted chimera were crossed to Flp recombinaseâexpressing mice to excise neomycin selection cassette. |
