| Primary Identifier | MGI:7526217 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Polr1a |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 71896885 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 71897931. The lacZ-neo cassette was removed through Flp-mediated recombination and subsequent Cre-mediated recombination removed the floxed exons, resulting in a knockout allele. |
