Primary Identifier | MGI:7520861 | Allele Type | Endonuclease-mediated |
Gene | Prm1 | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology generated an AAA to GCA change resulting in a lusine to alanine substitution at amino acid 49 (p.K49A). A few synonymous alterations were incorporated to introduce an FspI site for genotyping. The K49A mutation results in loss of positive charge as well as loss of acetylation at the site. |