| Primary Identifier | MGI:7522007 | Allele Type | Endonuclease-mediated |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<b-m2> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed using a modified Ai3 (Gt(ROSA)26So(tm3(CAG-EYFP)Hze)) vector where the eGFP sequence was replaced with coding regions for growth factor independent 1 transcription repressor (Gfi1) and atonal bHLH transcription factor 1 (Atoh1) fused to an enhanced green fluorescent protein (eGFP). Specifically, the targeting vector includes (from 5' to 3') a splice acceptor, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP (with stop codons in all 3 reading frames and a triple polyA signal) cassette, Gfi1 sequence, a GSG oligopeptide linker-P2A peptide sequence, Atoh1/eGFP fusion gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), polyA sequence, followed by an attB/attP-flanked PGK-neo-PGKpA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. |
