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Allele : Gt(ROSA)26Sor<em3(CAG-Gf1i,-Atoh1/EGFP,-Pou4f3)Akg> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 3, Andrew K Groves
Primary Identifier  MGI:7522011 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Inserted expressed sequence, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  129S7/SvEvBrd-Hprt1<b-m2>
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting vector is designed using a modified Ai3 (Gt(ROSA)26So) vector where the eGFP sequence was replaced with coding regions for growth factor independent 1 transcription repressor (Gfi1), atonal bHLH transcription factor 1 (Atoh1) fused to an enhanced green fluorescent protein (eGFP) and POU domain, class 4, transcription factor 3 (Pou4f3). Specifically, the targeting vector includes (from 5' to 3') a splice acceptor, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP (with stop codons in all 3 reading frames and a triple polyA signal) cassette, Gfi1 sequence, a GSG oligopeptide linker-P2A peptide sequence, Atoh1/eGFP fusion gene, a second GSG oligopeptide linker-P2A peptide sequence, Pou4f3 sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), polyA sequence, followed by an attB/attP-flanked PGK-neo-PGKpA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • Rosa26-GAP,
  • Rosa26-GAP
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1 Feature

Genome

3 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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1 Carried By

0 Driven By

3 Publication categories





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