Primary Identifier | MGI:7516772 | Allele Type | Transgenic |
Attribute String | Inducible, Knockdown | Gene | Tg(Pgk-tetR,tetO-RNAi:Pum3)#Yuj |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct containing a Pgk promoter driving a tetracycline repressor gene followed by a poly a sequence and a tetracycline responsive Pol III hybrid promoter driving expression of Pum3 shRNAs. Treatment with doxycycline results in about an 80% reduction in Pum3 mRNA expression in hemizygous embryos. |