| Primary Identifier | MGI:7543571 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Ighg1 |
| Transmission | Germline | Strain of Origin | C57BL/6NCrSlc |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Mouse IgG1 heavy chain constant region, exon 2 to exon 5, was replaced by human IgG4 (IGHG4) counterpart, exon 2 to exon 5, and a loxP-flanked neomycin selection cassette. The neomycin selection cassette was removed via cre-mediated recombination. Mouse IgG1 exon 1 was untouched and exons 6-7 were also untouched to preserve the mouse original intracellular signaling. Mouse IgG1 intron 5 was also left to preserve usual spicing. |
