| Primary Identifier | MGI:7514998 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Syngap1 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 endonuclease-mediated genome editing is used to create a single base substitution at c.3583-9 (G to A) that creates a cryptic splice acceptor site and premature stop codon in intron 16. The aberrant splicing results in a 7 base pair addition within mRNA transcripts.The mutation was confirmed by sequencing and Southern blot. |
