Primary Identifier | MGI:7529474 | Allele Type | Endonuclease-mediated |
Attribute String | Conditional ready, Epitope tag, Reporter | Gene | Gt(ROSA)26Sor |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/cas9 genome editing is used to insert a human EEF1a1 promoter followed by::Lox2272::LoxP::EGFP (inverted)::P2A::APEX-NES (inverted)::FLAG tag::Lox2272::LoxP::hGH double floxed inverted orientation cassette into intron 1 of Gt(ROSA)26Sor. APEX2 is an engineered monomeric peroxidase reporter derived from dimeric soybean ascorbate peroxidase. |