| Primary Identifier | MGI:7565401 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Swhtr |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cells for generating the allele are either G4 (129S6/SvEvTac x C57BL/6NTac)F1 or C57BL/6J (J:342171). |
| molecularNote | A FRT-flanked PGK-Neo-SV40pA selection cassette inserted a strong transcriptional stop signal (3xpA) downstream of a phylogenetically conserved region of the GATA4 bound first heart field specific enhancer. The selection cassette was removed via flp-mediated recombination. This insertion causes a premature termination of transcription and a severely shortened transcript. RNA-seq and qPCR confirmed the absence of full-length RNA in E9.5 hearts. The expression level of its cis located gene Nkx2-5 is unchanged in E9.5 hearts. And the lncRNAs IRENE-SS and IRENE-div expression levels are not affected. |
