| Primary Identifier | MGI:7571433 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Slc17a8 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting vector contains, from 5' to 3', a partial Slc17a8 sequence spanning intron 11, an frt3 site within Slc17a8 intron 11, a partial Slc17a8 exon 12 sequence up to and including the endogenous stop codon, an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 late polyA signal, and an AttP site. |
