| Primary Identifier | MGI:7562323 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Idh1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into intron 2, and a lox511 site, and, in reverse orientation, a copy of exon 3 (where arginine codon 132 (CGA) was changed to histidine (p.R132H)), a second loxP site and a second lox511 site, were inserted into intron 3. An FRT site flanked selection cassette that was inserted between the second loxP and lox511 sites was removed through subsequent Flp-mediated recombination. This allele expresses the wildtype peptide and only after conditional Cre-mediated flipping of the mutated exon copy and removal of the wildtype exon will it express the modified peptide. The mutation represents the most common variant found in human gliomas. |
