| Primary Identifier | MGI:7576205 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Ccdc28b |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology targeting the 5â end of exon 3 generated two one base pair deletions, and is predicted to result in a frameshift and premature stop codon. Exon 3 is shared by all transcripts and coding isoforms. Real-time qPCR showed a reduction in mRNA levels, likely due to NMD-mediated degradation of premature stop codon containing mRNA. Western blot analysis shows absence of protein. |
