Primary Identifier | MGI:7607007 | Allele Type | Endonuclease-mediated |
Attribute String | Endonuclease, Reporter | Gene | Sycp1 |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | Guide RNAs were designed to insert a cassette encoding, about 1200 bp of 5â Sycp1 sequence (5âHA), P2A self- cleaving peptide, codon-optimized Cas9 (hSPCas9), T2A self-cleaving peptide, eGFP coding sequence and about 1200 bp of 3â Sycp1 (3âHA) cleaving peptide, just before the stop codon. |