| Primary Identifier | MGI:7576958 | Allele Type | Targeted |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Snca |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed to insert a 158 kb (Chr4:89707185 - 89865027) fragment of the human SNCA carrying the A53T (alanine to threonine) mutation in place of Chr6:60683985-60848702 of the mouse Snca gene. The genomic sequences are from human assembly GRCh38.p14 and the mouse assembly GRCm39. The construct includes an loxP-flanked neomycin selection cassette. In addition, there is also a SNCA (non-coding antisense RNA) in the human genome segment. The insertion disrupts Snca transcription. Transient cre-medited recombination removed the neo cassette. |
