| Primary Identifier | MGI:7639701 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Zp3 |
| Strain of Origin | C57BL/6N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A T nucleotide was inserted after the first nucleotide of arginine codon 350 (CGA) and the last nucleotide was changed from A to T, changing it to leucine (CTG) and creating a frameshift and premature stop codon (TAA) immediately after (c.1049_1050delGAinsTGT:p.R350Lfs*2), using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation is the equivalent of a similar human mutation (NM_001110354.1:c.1045_1046insT:p.R349Lfs*2) associated with female infertility. |
