| Primary Identifier | MGI:7579305 | Allele Type | Targeted |
| Attribute String | Modified regulatory region | Gene | Rr468 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Nucleotides were changed (CACCTG to GAATTC and ACTGTACACAGGTGTTCA to CAGTGCTAGCTTGCAGAC) in the ebox1 and ebox2 sequences of this intronic enhancer downstream of Thrb B2 isoform (ENSMUST00000022304)-specific alternative exon 1 in intron 4 (B2 intron 1). Initially, for selection purposes, a self-excising ACN cassette (loxP site + testis-specific ACE promoter driven cre gene + neomycin resistance gene cassette + loxP site) was inserted into intron 4. Subsequently, this cassette was self-deleted during breeding of chimeras, leaving only a single loxP site remaining at the boundary upstream of the enhancer |
