| Primary Identifier | MGI:7621414 | Allele Type | Targeted |
| Gene | Chrna2 | Transmission | Germline |
| Strain of Origin | 129S4/SvJae | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Leucine codon 282 (CTC) in exon 5 was changed to serine (AGC) (p.L282S) and an FRT site flanked neomycin resistance gene cassette was inserted into intron 4. The neo cassette was removed through subsequent Flp-mediated recombination. The mutation, at Leu 9' in the transmembrane region 2 (TM2) of the encoded peptide, modifies the physiological response of the receptor to nicotine and acetylcholine. |
