| Primary Identifier | MGI:7593769 | Allele Type | Targeted |
| Gene | E2f3 | Transmission | Germline |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Leucine codon 430 (CTG) in exon 7 was changed to glutamine (CAG) (p.L430Q) and a loxP site flanked neomycin resistance gene cassette was inserted into intron 6. The neo cassette was removed through subsequent Cre-mediated recombination. The mutation, in the highly conserved Rb-binding domain of the encoded peptide, blocks retinoblastoma-family-protein-binding. |
