| Primary Identifier | MGI:7624036 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Egln3 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted into intron 2 and a loxP2272 site, in inverted copy of exon 3 (where histidine codon 196 (CAT) was changed to alanine (GCT) (p.H196A)), a second loxP site, a second loxP2272 site, and an FRT site flanked neomycin resistance gene cassette, were inserted into intron 3. The neo cassette was removed through subsequent Cre-mediated recombination. This allele expresses the wildtype peptide. Only after Cre-mediated removal of the endogenous exon 3 and flipping of the mutated copy, will it express the mutated peptide. The mutation abolishes the prolyl hydroxylase activity of the encoded peptide. |
