| Primary Identifier | MGI:7615147 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cdkn2a |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A DNA construct encoding a fluorescent reporter protein (tdTomato) was inserted into the endogenous first exon (exon 1alpha) of the p16/INK4apart of the gene through homologous recombination. The insertion knocks out expression of the p16/INK4a transcript and does not affect expression of the Arf transcript. A stop codon and poly-A signal were included at the end of the inserted reporter gene. An FRT-flanked neomycin selection cassette was also inserted into the first intron, and is retained in this allele. |
