| Primary Identifier | MGI:7641520 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Otud3 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 2 and an FRT-flanked neomycin selection cassette and second loxP site were inserted downstream of exon 2. The selection cassette was removed via flp-mediated recombination. Exon 2 was deleted via cre-mediated recombination in the germline. The frame-shift caused by lack of exon 2 produces stop codons in exon 3. Western blot analysis confirmed absence of protein. |