| Primary Identifier | MGI:7641935 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Tbk1 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Genomic sequence (230 bp) containing sequences of the 3' end of intron 4 and the 5' end of exon 5 was replaced with a loxP site, the 3' end of a CMV intron, a cDNA coding for exons 5-21, a 4x poly(A) signal sequence, an FRT site flanked neomycin selection marker cassette, a second loxP site, and 5' exon 5 sequence where aspartic acid codon 135 (GAT) was changed to asparagine (p.D135N). The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wildtype transcript and only after Cre-mediated removal of the wildtype cDNA sequence will it express the mutated transcript. The mutation renders the encoded peptide catalytically inactive. |
