| Primary Identifier | MGI:7645903 | Allele Type | Endonuclease-mediated |
| Gene | Gars1 | Strain of Origin | B6;CAST-Gars1<Nmf249>/RwbJ |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using oocytes that contain the Gars1Nmf249 allele (where proline codon 278 (CCT) is replaced with tyrosine and lysine codons (AAATAT) (p.P278delinsKY)), leucine codon 88 (CTC) in exon 2 was changed to alanine (GCC) (p.L88A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutated residue is located in the WHEP domain of the encoded peptide. |
