Primary Identifier | MGI:7660528 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Slc13a3 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The L1L2_Bact_P cassette was inserted at position 165287182 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 165288050. The critical exon(s) is/are thus flanked by loxP sites. The lacZ and neomycin resistance gene were deleted via flp-mediated recombination creating a âconditional readyâ floxed allele. |