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Allele : Piezo2<em1.1Ddg> piezo-type mechanosensitive ion channel component 2; endonuclease-mediated mutation 1.1, David D Ginty
Primary Identifier  MGI:7719842 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag, Reporter Gene  Piezo2
Transmission  Germline Strain of Origin  (129S6/SvEvTac x C57BL/6J)F1
Is Recombinase  false Is Wild Type  false
description  ES cell line = G1, established from a blastocyst created by crossing a 129S6 mouse with a C57BL/6J mouse.
molecularNote  Using Crispr/cas9 system, a FLAG-tagged spaghetti monster fluorescent protein (smFP-FLAG) was fused to the 3' end of mouse Piezo2 exon 54 and followed by an FRT-flanked neomycin cassette. smFP-FLAG encodes 10 FLAG peptides and an intact green fluorescent protein (GFP) chromophore. After achieving successful germline transmission, the frt-NeoR-frt cassette was excised.
  • mutations:
  • Insertion
  • synonyms:
  • Piezo2<smFP-FLAG>,
  • Piezo2<smFP-FLAG>
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Trail: Allele

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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