Primary Identifier | MGI:7714642 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Pten |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6J)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A furin cleavage linker-T2A-EYFP-FRT-PGK-Neomycin/Kanamycin-bGH pA-FRT cassette was inserted immediately upstream of the stop codon in exon 9 (the last exon) of the mouse Pten gene via homologous recombination. The FRT-flanked drug selection cassette was excised by crossing with R26 FLP mice. |