| Primary Identifier | MGI:7861921 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Cd1d2 |
| Inheritance Mode | Not Specified | Strain of Origin | BALB/cAnNRj |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | CRISPR-targeting of exon 1 deleted a T at position 23, inducing a downstream STOP codon. Thus, from the start codon ATG to the new STOP, a 38-aa protein was produced. CRISPR technology details: Cas9 mRNA and sgRNAs targeting exon1 were mixed and electroporated (NEPA21) into the pronuclei of the one-cell embryos. SgRNA2 5â²-CCTACCGTGCCTGTTGCTGT-3â² and SgRNA4 5â²-CAGCAACAGGCAC GGTAGGT-3â². |
