Primary Identifier | MGI:7790913 | Allele Type | Endonuclease-mediated |
Attribute String | Modified regulatory region | Gene | Rr590 |
Strain of Origin | C57BL/6 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | The Cyp27b1 enhancer, located in an Eef1akmt3 (Mettl21b) intron, was targeted using sgRNAs (equivalent to CCTACCTTCCCGCTACTGTTGGG and GCATGCAATGGCCCTCCGTTTGG) with CRISPR/Cas9 technology, resulting in a 914 bp deletion (GRCm39:chr10:126868979-126869892) that includes the start of the coding last Eef1akmt3 exon. |