Primary Identifier | MGI:7666515 | Allele Type | Endonuclease-mediated |
Attribute String | Modified regulatory region | Gene | In(13Rr494;Rr493)1Andg |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A section of genomic sequence, containing Pitx1 enhancer RA4, the Macroh2a1 gene and Pitx1 pan-limb enhancer Pen, was inverted using sgRNAs (equivalent to AACAAGATGATACCGCATGG and CGTGCTATCGAGGGACTAAT) with CRISPR/Cas9 technology. |