Primary Identifier | MGI:7703763 | Allele Type | Endonuclease-mediated |
Attribute String | Modified regulatory region | Gene | Rr112510 |
Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The CTCF-binding site located in Lmbr1 intron 4, was deleted using an sgRNA with CRISPR/Cas9 technology in ESCs that carry the Rr112507em1Andg CTCF-binding site deletion allele. This allele represents two different deletions (CTTCTACTGCCACCTGCTGGTATCAC on one copy of chromosome 5 and CTGGCTCACCATAGAGTTATCTTCTACTGCCACCTGCTGGTATCACAAAATAGTTCAAAATA on the other) that contain the binding site CTGCCACCTGCTGGTATCA. |