| Primary Identifier | MGI:7665326 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Cab39l |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 59743287 of Chromosome 14 upstream of the critical exon 6 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 6 at position 59744101. The critical exon 6 is thus flanked by loxP sites. Flp-medated recombination removed the lacZ and neomycin resistance gene, generating a âconditional readyâ exon 6 floxed allele. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml |
