| First Author | Vacaru AM | Year | 2013 |
| Journal | Genesis | Volume | 51 |
| Issue | 11 | Pages | 751-62 |
| PubMed ID | 23913596 | Mgi Jnum | J:351560 |
| Mgi Id | MGI:7702386 | Doi | 10.1002/dvg.22420 |
| Citation | Vacaru AM, et al. (2013) Analysis of primitive erythroid cell proliferation and enucleation using a cyan fluorescent reporter in transgenic mice. Genesis 51(11):751-62 |
| abstractText | Primitive erythropoiesis is a vital process for mammalian embryonic development. Here we report the generation and characterization of a new transgenic mouse line that expresses a histone H2B-CFP fusion protein in the nuclei of primitive erythroid cells. We demonstrate the potential of this epsilon-globin-histone H2B-CFP line for multicolor imaging and flow cytometry analysis. The epsilon-globin-H2B-CFP line was used to analyze the cell cycle distribution and proliferation of CFP-expressing primitive erythroblasts from E8.5-E13.5. We also evaluated phagocytosis of extruded CFP-positive nuclei by macrophages in fetal liver and placenta. The epsilon-globin-H2B-CFP transgenic mouse line adds to the available tools for studying the development of the primitive erythroid lineage. |
