| Primary Identifier | MGI:7781825 | Allele Type | Endonuclease-mediated |
| Gene | Prune1 | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was generated at the Institut Clinique de la Souris by co-electroporating Cas9 protein, a crRNA (CTTACTTGGGTAAAATATGG)/tracrRNA and an ssODN template, which resulted in a p.D106N mutation in exon 3 (ENSMUSE00000253496; GRCm39). A DdeI diagnostic restriction site was introduced to facilitate genotyping. |
