Primary Identifier | MGI:7666562 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Col1a1 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | ES cells containing the Col1a1tm13(neo/hygro*)Jae allele were targeted, using Flp recombinase, with a vector to replace the FRT site flanked neomycin resistance gene cassette in the homing cassette downstream of Col1a1 with an FRT site flanked cassette containing putative Pitx1 enhancer sequence B (GRCm39:chr13:56258927-56261815 in intron 2 of Macroh2a1) followed by the beta-galactosidase reporter gene lacZ, PGK promoter sequence and ATG start site. |