| First Author | Ge T | Year | 2024 |
| Journal | Biol Reprod | Volume | 110 |
| Issue | 5 | Pages | 877-894 |
| PubMed ID | 38236177 | Mgi Jnum | J:351297 |
| Mgi Id | MGI:7660995 | Doi | 10.1093/biolre/ioae012 |
| Citation | Ge T, et al. (2024) Coiled-coil domain containing 159 is required for spermatid head and tail assembly in mice. Biol Reprod 110(5):877-894 |
| abstractText | The centrosome is critical for maintaining the sperm head-tail connection and the formation of flagellar microtubules. In this study, we found that in mouse testes, CCDC159 (coiled-coil domain-containing protein 159) is specifically localized to the head-tail coupling apparatus (HTCA) of spermatids, a structure that ensures sperm head-tail tight conjunction. CCDC159 contains a C-terminal coiled-coil domain that functions as the centrosomal localization signal. Gene knockout (KO) of Ccdc159 in mice resulted in acephalic spermatozoa, abnormal flagella, and male infertility. To explore the mechanism behind CCDC159 regulating spermatogenesis, we identified CCDC159-binding proteins using a yeast two-hybrid screen and speculated that CCDC159 participates in HTCA assembly by regulating protein phosphatase PP1 activity. Further RNA-sequencing analyses of Ccdc159 KO testes revealed numerous genes involved in male gamete generation that were downregulated. Together, our results show that CCDC159 in spermatids is a novel centrosomal protein anchoring the sperm head to the tail. Considering the limitation of KO mouse model in clarifying the biological function of CCDC159 in spermatogenesis, a gene-rescue experiment will be performed in the future. |
