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Allele : En2<em1Alj> engrailed 2; endonuclease-mediated mutation 1, Alexandra L Joyner

Primary Identifier	MGI:7789004	Allele Type	Endonuclease-mediated
Attribute String	Epitope tag	Gene	En2
Strain of Origin	Not Specified	Is Recombinase	false
Is Wild Type	false

molecularNote

Using CRISPR technology, a single-stranded oligodeoxynucleotide (ssODN) was designed to insert a linker (GlyLysSerAspSerGlu) and three consecutive HA tags (3xHA) in-frame into the C-terminal of the En2 gene, replacing the endogenous stop codon.

mutations:
Insertion

synonyms:
En2-HA,
En2-HA

earliestPublication:
Willett RT, et al. (2019) Cerebellar nuclei excitatory neurons regulate developmental scaling of presynaptic Purkinje cell number and organ growth. Elife 8:e50617

1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

Other

1 Carried By

0 Driven By

3 Publication categories

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