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Allele : Gt(ROSA)26Sor<tm11(Igk-V/Igh-V)Ics> gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Mouse Clinical Institute

Primary Identifier  MGI:7788428 Allele Type  Targeted
Attribute String  Conditional ready, Inserted expressed sequence, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  C57BL/6NTac
Is Recombinase  false Is Wild Type  false
molecularNote  The following elements were inserted into an intron: an adenovirus splice acceptor site, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK and 3x SV40 p(A) signal sequences), followed by the Igk signal peptide sequence, anti-En1 and En2 Igk-V/Igh-V single chain variable fragment (scfv) antibody cDNA with 6x Myc- and His-tag sequence in-frame, P2A self-cleaving sequence, the EGFP reporter gene, and SV40 poly(A) signal sequence. This allele only expresses the antibody and EGFP after Cre-mediated removal of the stop cassette.
  • mutations:
  • Insertion
  • synonyms:
  • Gt(ROSA)26Sortm11(LSL-anti-En1-2scFv-P2A-eGFP)Ics,
  • Gt(ROSA)26Sortm11(LSL-anti-En1-2scFv-P2A-eGFP)Ics
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

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0 Driven By

3 Publication categories