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Allele : Galm<tm2d(EUCOMM)Hmgu> galactose mutarotase; targeted mutation 2d, Helmholtz Zentrum Muenchen GmbH

Primary Identifier  MGI:7738544 Allele Type  Targeted
Attribute String  Null/knockout Gene  Galm
Transmission  Germline Strain of Origin  C57BL/6N-A<tm1Brd>
Is Recombinase  false Is Wild Type  false
Project Collection  EUCOMM
molecularNote  The L1L2_Bact_P cassette was inserted at position 80444917 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39) exon 2. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) exon2 at position 80445851. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele with exon 2 floxed. Subsequent cre expression resulted in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
  • mutations:
  • Intragenic deletion,
  • Insertion
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

3 Publication categories