| Primary Identifier | MGI:7738844 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Exd2 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 genome editing technology was used to generate a deletion of the 3â² end of exon 2 and part of the downstream intron. The deleted region covers the DCE motif known to be critical for the 3â --> 5â exonuclease activity on both DNA and RNA. Sanger sequencing of small testicular biopsies demonstrated a deletion of a 14-nucleotide region at the 3â end of exon 2. Analysis of RNA-seq libraries showed that the deletion results in aberrant splicing of exon 2 resulting in absence of 3â end of exon 2 or whole exon 2 skipping. The most common splicing pattern results in an in-frame deletion of 33 amino acids, including the DCE motif. |
